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Construction and expression of pGEX-e23(scFv) PE40 in Escherichia coli

Hui Wang, Wei Han, Zhen Yan, Yingqi Zhang

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Abstract

e23 (scFv) PE40 fusion gene for prokaryotic expression was constructed and inserted into a prokaryotic fusion protein expresson vector pGEX-4T and expressed in Escherichia coli DH5alpha. The DNA sequence of e23 (scfv) PE40 fusion gene was correct. A new anticipated M_(r) 90 000-protein band appeared on SDS-PAGE gel induced by IPTG and amounted to 15% of the total bacterial protein.

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What this paper is about

e23 (scFv) PE40 fusion gene for prokaryotic expression was constructed and inserted into a prokaryotic fusion protein expresson vector pGEX-4T and expressed in Escherichia coli DH5alpha. The DNA sequence of e23 (scfv) PE40 fusion gene was correct. A new anticipated M_(r) 90 000-protein band appeared on SDS-PAGE gel induced by IPTG and amounted to 15% of the total bacterial protein.

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Available abstract

e23 (scFv) PE40 fusion gene for prokaryotic expression was constructed and inserted into a prokaryotic fusion protein expresson vector pGEX-4T and expressed in Escherichia coli DH5alpha. The DNA sequence of e23 (scfv) PE40 fusion gene was correct. A new anticipated M_(r) 90 000-protein band appeared on SDS-PAGE gel induced by IPTG and amounted to 15% of the total bacterial protein.

Key concepts: Escherichia coli, Fusion protein, lac operon, Molecular biology, Biology, Gene, Fusion gene, Microbiology

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Construction and expression of pGEX-e23(scFv) PE40 in Escherichia coli — Research Paper | ScholarLens