2000•Biotechnology(Faisalabad)Requires access
Cloning and expression of endostatin in escherichia coli
WU Cheng-jun, Lijian Ye, Xiaozhong Peng, Zhigang Ma, Jimei Liu, Ningzhu Hu, Bo Yan, Xingqi Huang
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Abstract
The total RNA was extracted from the fetal liver and the endostatin gene was amplifieated by RT-PCR. After identified by DNA sequencing, it was cloned into the expression vector pGEX-KG and then transformed into the host cells E. coli DH5alpha and BL21. Subsequently the recombinant GST-fusion protein was expressed and purified.