Cloning and Expression of Stress Associated Protein Gene from Banana(Musa acuminata L.AAA group,cv.Brazilian)

The aim of this paper is to acquire genes related to the stress response in banana.A stress associated protein gene was acquired from banana roots cDNA library by randomly sequencing,which was named MaSAP1(GenBank accession number:AGH14257.1).The sequence amplified from banana root cDNA was the same with fragment sequences of plasmid OZ092 in library,indicating that MaSAP1 was a fulllength cDNA,containing a 510bp of the largest open reading frame,encoding 169 amino acids.The homology alignment of protein sequence found to contain complete A20 and AN1 motif.Phylogenetic analysis indicated that the MaSAP1 was closer with Oryza sativa and Aeluropus littoralis.Tissue-specific expression study showed that the expression of MaSAP1 was higher in the roots and fruits,the lowest in the stems. Real-time PCR analysis showed MaSAP1 in response to hormone treatment,and also in response to various stress,such as drought,low temperature,high salt and FocTR4.Altogether the results in this work demonstrated that MaSAP1 may play an important biological role in plant growth and development,and plant response to different stress types.