Molecular Cloning and Expression Analysis of MaOPR Gene from Banana

Through RACE approaches and bioinformatics analysis,the full-length cDNA of the cysteine synthase gene,named as MaOPR(12-oxo-phytodienoic acid reductase),was obtained from root of banana(Musa acuminata L.AAA group'Brazilian')cDNA library,and its sequence characters were also analyzed.The full length of this gene was 1 512 bp,it had an 1 287 bp open reading frame in length encoding 429 amino acid.The result of bioinformatics showed that this protein was a stable protein with the active sites,the matrix attachment sites and the flavin mononucleotide binding sites,which pI is 8.11.The sequence prediction showed that it located in the cytoplasm,it was not a transmembrane protein and had no signal peptide.Compared with other known plants,the homology of MaOPR was more than 66%.Amino acids homology analysis indicated that MaOPR had 71% similarity compared with Medicago sativa,Setaria italic,Oryza sativa,Astragalus sinicus.RT-PCR analysis showed that MaOPR was constitutively expressed in roots,stems,leaves,flowers and fruits.The expression level was the highest in fruits.Analysis showed that MaOPR responsed to the stress including the ABA inhibitor,ethylene and wilt disease stress.