Cloning and Expression Analysis of a New Stress-resistant ERF Gene from Banana

A full length cDNA named MaERF which was cloned from banana on the basis of a AP2/ERF fragment combined with RACE technology. The full cDNA of this gene was 1 611 bp,the open reading frame was 1 128 bp and coded a polypeptide of 376 amino acids. This gene had one typical AP2/ERF domain. The genome sequence full-length of this gene was 2 881 bp,and contained one intron splice site according to the rules of theGT-AG. The phylogenetic tree shows that the gene belongs to B2 subsets of ERF genes,and they are more involved in plant stress reactions. Semi-quantitative RT-PCR analysis showed that the gene was expressed in various organs of the banana,with lower the amount of expression in the roots and leaves,while high expression in fruit and flowers;Fluorescence quantitative real-time PCR detection showed this gene had high expression with Fusarium oxysporum and low temperature stress,so this gene may play an important role in bananas stress reaction.