Application of orthogonal design in tissue culture of Cerasus campanulata(Rosaceae)

Effects of 6-BA,NAA,2,4-D and different explants on callus induction and the inducting rate of fasciculated shoots in Cerasus campanulata were studied.Statistic analysis indicated that the main factor which influenced callus induction and the inducting rate of fasciculated shoots was explant.The optimal medium for callus induction was MS+1.0mg·L-1 BA+1.0mg·L-1 NAA+0.5mg·L-1 2,4-D+Leaves,the inducing rate was 94.5%;the optimal medium for the inducting rate was MS+1.0mg·L-1 6-BA+0.5mg·L-1 NAA+stem section with bud,and the inducing rate was 37.5%.The medium of MS with 1.0mg·L-1 BA and 0.05mg·L-1 NAA was optimal for multiplication,and the multiplication rate was 460%.Plantets were transplanted to the 1/2MS medium with 0.8mg·L-1 IBA and 0.2mg·L-1 NAA for rooting,when the explants reached 2cm,the rooting rate could reach 90% after 30 days,and the suvival rate of transplant was 92%.