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2005•Journal of Chinese Integrative MedicineRequires access

Determination of baicalin in Xiaoyanling Decoction by high- performance liquid chromatography

Jiang Jun

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Abstract

Objective: To establish a high-performance liquid chromat ography (HPLC) method for the determination of baicalin in Xiaoyanling Decoc tion. Methods: HPLC method was used for determination. A Kromo sil C 18 column was used. The mobile phase consisted of MeOH-acid water (H 2O∶H 3PO 4=53∶0.2) 40∶60, and the detective wavelength was 277 nm. Results: The content of baicalin in each Xiaoyanling D ecoction sample was not less than 0.40 mg/ml (RSD5%). Conclusion: The content of baicalin in Xiao- yanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

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What this paper is about

Objective: To establish a high-performance liquid chromat ography (HPLC) method for the determination of baicalin in Xiaoyanling Decoc tion. Methods: HPLC method was used for determination. A Kromo sil C 18 column was used. The mobile phase consisted of MeOH-acid water (H 2O∶H 3PO 4=53∶0.2) 40∶60, and the detective wavelength was 277 nm. Results: The content of baicalin in each Xiaoyanling D ecoction sample was not less than 0.40 mg/ml (RSD5%). Conclusion: The content of baicalin in Xiao- yanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

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Available abstract

Objective: To establish a high-performance liquid chromat ography (HPLC) method for the determination of baicalin in Xiaoyanling Decoc tion. Methods: HPLC method was used for determination. A Kromo sil C 18 column was used. The mobile phase consisted of MeOH-acid water (H 2O∶H 3PO 4=53∶0.2) 40∶60, and the detective wavelength was 277 nm. Results: The content of baicalin in each Xiaoyanling D ecoction sample was not less than 0.40 mg/ml (RSD5%). Conclusion: The content of baicalin in Xiao- yanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

Key concepts: Baicalin, Decoction, High-performance liquid chromatography, Chromatography, Chemistry, Traditional medicine, Medicine

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