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Experimental study on Jurkat cell apoptosis induced by Boswellia carterii Birdw extractive

Zhenhua Qi

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Abstract

Objective: To study the influence of Boswellia carteriiBirdw(BCB) extractive of different concentrations on human Jurkat cell apoptosis at different time points. Methods: Agarose gel electrophoresis, transmission electron microscope(TEM) and flow cytometry(FCM) were used for observing DNA ladders, morphology of Jurkat cells and cell cycle, respectively. Results: BCB induced apoptosis of Jurkat cells and typical DNA ladders; TEM demonstrated the presence of apoptosis Jurkat cells, condensation of cytoplasm, numerous vaculoes in cytoplasm, compaction of the nuclear chromatin and formation of apoptosis dody. The subG 1 peak was detected by cell cycle analysis. It revealed that G 1 phage cells increased and S phage cells decreased. Conclusion: The data indicate that BCB extractive induces time and concentration dependent apoptosis in Jurkat cell line. [

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What this paper is about

Objective: To study the influence of Boswellia carteriiBirdw(BCB) extractive of different concentrations on human Jurkat cell apoptosis at different time points. Methods: Agarose gel electrophoresis, transmission electron microscope(TEM) and flow cytometry(FCM) were used for observing DNA ladders, morphology of Jurkat cells and cell cycle, respectively. Results: BCB induced apoptosis of Jurkat cells and typical DNA ladders; TEM demonstrated the presence of apoptosis Jurkat cells, condensation of cytoplasm, numerous vaculoes in cytoplasm, compaction of the nuclear chromatin and formation of apoptosis dody. The subG 1 peak was detected by cell cycle analysis. It revealed that G 1 phage cells increased and S phage cells decreased. Conclusion: The data indicate that BCB extractive induces time and concentration dependent apoptosis in Jurkat cell line. [

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Available abstract

Objective: To study the influence of Boswellia carteriiBirdw(BCB) extractive of different concentrations on human Jurkat cell apoptosis at different time points. Methods: Agarose gel electrophoresis, transmission electron microscope(TEM) and flow cytometry(FCM) were used for observing DNA ladders, morphology of Jurkat cells and cell cycle, respectively. Results: BCB induced apoptosis of Jurkat cells and typical DNA ladders; TEM demonstrated the presence of apoptosis Jurkat cells, condensation of cytoplasm, numerous vaculoes in cytoplasm, compaction of the nuclear chromatin and formation of apoptosis dody. The subG 1 peak was detected by cell cycle analysis. It revealed that G 1 phage cells increased and S phage cells decreased. Conclusion: The data indicate that BCB extractive induces time and concentration dependent apoptosis in Jurkat cell line. [

Key concepts: Jurkat cells, Apoptosis, Flow cytometry, Cytoplasm, Cell cycle, Molecular biology, Cell, Agarose gel electrophoresis

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Experimental study on Jurkat cell apoptosis induced by Boswellia carterii Birdw extractive — Research Paper | ScholarLens