2002•Fujian Yike Daxue xuebaoRequires access

Apoptotic effect of CD_(154) antisense RNA on Jurkat cell

Xiang-xiong Zheng, Jian Ding, Xuwei Yang

Abstract

CD_(154 gene extracellular domain sequence was amplified by RT-PCR. Then, the PCR product was cloned and subcloned with T-A cloning and subcloning techniques. After the vector was transfected into Jurkat cells, apoptotic data were obtained with flow cytometry (FCM) and electron microscope techniques. Apoptotic bodies were found in the transfeced Jurkat cells under electron microscopel; and FCM analysis showed a significant apoptotic peak.

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Available abstract

Key concepts: Jurkat cells, Subcloning, Molecular biology, Transfection, Apoptosis, Flow cytometry, Cloning (programming), Extracellular