1998•Europe PMC (PubMed Central)Requires access
Preparation of recombinant retrovirus vectors containing human interleukin-2 gene and its transfection into leukemia cells
Fang Yin, Baisong Wu, Qifa Liu, Weiyang Zheng, Bin Wu, Shu-Yun Zhou
Open publisher page 0 citations
Abstract
Recombinant retrovirus vectors containing human IL-2 gene were packaged with PA317 packaging cells. The supernatants containing recombinant retroviruses were used to infect leukemia cell lines K562 and HL-60. The presence of neomycin-resistant (neoR) gene was confirmed by Southern blot and the IL-2 activities were measured by MTT method. Thus human IL-2 gene was successfully transfected into K562 and HL-60 cells. The results showed that the leukemia cells transfected with human IL-2 gene grew much more slowly than those untransfected or transfected with control vectors. The agar colonogeneicity assay also showed that the colony number of IL-2 gene-transfected cells was much less than that of untransfected cells or cells transfeced with control vectors. Therefore, the proliferation activity of leukemia cells transfected with IL-2 gene was reduced.