Effects of annexin II antisense vector on the growth of lung cancer cells

The total RNA was isolated from human lung cancer cell line SPC-A-1 and the target DNA fragments were amplified by RT-PCR. The antisense expression vector was constructed by double restriction endonuclease cleavage directional clone method. Annexin II antisense expression vector was introduced into SPC-A-1 cells by liposome transfection reagent. The expression of annexin II mRNA was analyzed by semi-quantitative RT-PCR. The effects of antisense vector of annexin II gene on the growth of SPC-A-1 were observed. The antisense vector of annexin II gene was constructed and introduced into SPC-A-1 cells successfully. Semi-quantitative RT-PCR showed that the annexin II mRNA expression reduced by about two thirds in the transfected cells as compared with that in the untrasfected cells. Compared with the untransfected cells, transfected cells decreased significantly in cell growth, clone formation efficiency in plating and DNA synthesis. Cell cycle was blocked in G_(0)-G_(1) phase. Annexin II could promote the growth of lung cancer cells and may be helpful for the development of lung cancer.