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IMMUNOSCREENING OF cDNA CLONES EXPRESSING SCHISTOSOMA JAPONICUM ANTIGENS BY SERUM FROM REINFECTED PEOPLE

Wan Rongzh

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Abstract

To obtain valuable Schistosoma japonicum(Sj) antigen clones, an constructed Sj adult worm cDNA libray was screened with serum from reinfected people of Sj endemic area with high level Sj specific IgG4 antibodies.Then positive clones were preliminarily identified by PCR.Of the 8.6×104 phage plaques immunoscreened by human specific IgG4 antibody, 11 are positive with the PCR products' size ranged from 564bp to 740bp.

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What this paper is about

To obtain valuable Schistosoma japonicum(Sj) antigen clones, an constructed Sj adult worm cDNA libray was screened with serum from reinfected people of Sj endemic area with high level Sj specific IgG4 antibodies.Then positive clones were preliminarily identified by PCR.Of the 8.6×104 phage plaques immunoscreened by human specific IgG4 antibody, 11 are positive with the PCR products' size ranged from 564bp to 740bp.

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Available abstract

To obtain valuable Schistosoma japonicum(Sj) antigen clones, an constructed Sj adult worm cDNA libray was screened with serum from reinfected people of Sj endemic area with high level Sj specific IgG4 antibodies.Then positive clones were preliminarily identified by PCR.Of the 8.6×104 phage plaques immunoscreened by human specific IgG4 antibody, 11 are positive with the PCR products' size ranged from 564bp to 740bp.

Key concepts: Immunoscreening, Schistosoma japonicum, Biology, Antigen, Antibody, Complementary DNA, Virology, Schistosoma

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IMMUNOSCREENING OF cDNA CLONES EXPRESSING SCHISTOSOMA JAPONICUM ANTIGENS BY SERUM FROM REINFECTED PEOPLE — Research Paper | ScholarLens