Effects of Bisphenol A on Rat Sertoli Cell Function and Its Mechanism

Objective To investigate the effects of bisphenol A on SD rat Sertoli cell function and the injury mechanism. Methods SD rat Sertoli cells were treated with bisphenol A at different doses,and the control group,solvent control group were set. Sertoli cell proliferation,cell cycle and PCNA,Vimentin protein expression. were observed. Results The survival rate of Sertoli cells in the treated groups was significantly lower than the control group (P0.05),constitution ratio of G0/G1 phase cells increased,while for S phase and M-phase, it decreased,and PI decreased. Immunocytochemistry showed PCNA expression in Sertoli cells was significantly inhibited by 10-4,10-5 mol/L BPA. Vimentin expression in Sertoli cells was significantly inhibited by 10-4,10-5,10-6 mol/L BPA. Conclusion Bisphenol A can affect Sertoli cell proliferation,cell cycle and inhibit PCNA,Vimentin protein expression in Sertoli cells.