Cultivation of rat sertoli cell in vitro and effect of FSH on its proliferation

Rat sertoli cells were isolated and cultured in vitro,and effects of FSH on the prolifera- tion of the cultured sertoli cells were investigated.Tripsin and collagenase were used for harvesting and processing of the sertoli cell,and then the purity of sertoli ceils was identified by the immun- ofluorescence of Fas-L.Following culture of the above-prepared cells for 2 days,sertoli cells were incubated with the presence of the different concentration of FSH,after culture for 16 h,the prolif- eration of sertoli cells was determined by MTT assay.Results show the culture method is effective for sertoli cells of high purity;FSH has significantly stimulated sertoli cell proliferation(P0.01). The conclusion could contribute to the cultivation of rat sertoli cell in vitro.