2007•Jilin Nongye Daxue xuebaoRequires access

Analysis of Polymorphism on SLA-DQA Gene by PCR-RFLP in Min Pig

Dongjie Zhang

Abstract

Scanning and analysis of polymorphism on whole coding region of SLA-DRB gene were per- formed by cloning and PCR-RFLP method.The result showed that the exon 2 is a high changing region, mutation rate reached 5.6%,therein 80% is effective mutation,but no new allele was found.After genotyping by PCR-RFLP with Eco RⅠin exon2 3 genotypes were obtained:254 bp(AA),2.54 bp/ 88 bp/166 bp(AB),88 bp/166 bp(BB),with PvuⅡ3 genotypes were obtained:254 bp(AA), 254 bp/129 bp/125 bp(AB),129 bp/125 bp(BB),with FbaⅠin exon3 3 genotypes were obtained: 282 bp(AA),282 bp/130 bp/152 bp(AB),130 bp/152 bp(BB),with Hin1Ⅰin exon 4 3 genotypes were obtained:184 bp(AA),184 bp/83 bp/101 bp(AB),83 bp/101 bp(BB).Min pig is balanced in Eco RⅠsite(P0.05),but unbalanced in PvuⅡsite(P0.05) and significantly unbalanced in FbaⅠand Hin 1Ⅰsite(P0.01) by Hardy-Weinberg Equilibrium analysis.

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Available abstract

Key concepts: Genotyping, Restriction fragment length polymorphism, Genotype, Exon, Genetics, Molecular biology, Biology, Polymorphism (computer science)