Study on SNPs on part sequence of yak CAST gene

CAST was investigated as a potential candidate gene for a quantitative trait locus(QTL)affecting meat tenderness.In this study,two pairs of specific primers were designed to analyse CASTgene part of intron 2,exon 3 and part of intron 3 and exon 8,part of intron 8by PCR-SSCP technique.The sequencing results showed that a sequence of CTTTTTT absence was detected at 40 400bp in intron 2,the genotype frequencies of AA,AB and BB were 0.7514,0.2265 and 0.0221,and the allelic frequencies of A and B were 0.8177 and 0.1823 respectively.Two bases transformation were detected in exon 3,they were G→A at 40 455bp and A→G at 40 463bp,the genotype frequencies of AA and AB at the two sites were 0.7293 and 0.2707,and the A and B allelic frequencies were 0.8646 and 0.1354.And a base transformation of A→G in intron 8was detected.The allelic frequencies of A and B were 0.8177 and 0.182 3,and the genotype frequencies of AA and AB were 0.6354 and 0.3646 respectively.The gene heterozygosity(He)at 40400bp,40455bp,40463bp and 85170bp were 0.2265,0.2707,0.2707 and 0.299 0respectively;and the gene polymorphism information content(PIC)were 0.2067,0.2067,0.2067 and 0.2537 respectively.The population of yak was Hardy-Weinberg equilibrium at 40 400bp,but was not at 40455bp,40 463pb and 85 170bp.