Cloning and Polymorphism Analysis of VIPR-1 Gene in Black Muscovy Duck

As a critical candidate gene of poultry broodiness,the length of 1 864 bp(target 1) and 1 673 bp(target 2) gene fragments of the vasoactive intestinal peptide-1 gene were amplified with polymerase chain reaction method from the genomic DNAs Abstracted from the blood of black Muscovy duck.The results of sequence analysis demonstrated that the target fragment 1 included the complete sequence of the 5th exon(101 bp),the 5th intron(1 630 bp) and the 6th exon(133 bp).The target fragment 2 contained the complete sequence of the 12th exons(42 bp),the 12th introns(1 455 bp) and partial sequence of the 13th exons(176 bp).Another four pairs of specific primers(P1-P8) were designed according to the two sequences fragments,respectively.The results showed that the target fragment 1 had C/T mutation at position 318 bp and 454 bp,A/G mutation at 547 bp,A/T mutation at 923 bp;the target fragment 2 had C/T mutation at 89 bp and 944 bp,A/G mutation at 662 bp and 1 031 bp,A/C mutation at 1 334 bp.The characterization and mutations discovery with the VIPR-1 gene provided the basic evidences for the further study on the association of its polymorphisms with broodiness trait with the black Muscovy ducks.