Effects of transfected SOCS3 gene on expression of c-fos and c-jun mRNA and proliferation of A549 cells

A549 cells were cotransfected with pEFSOCS3 and pSV2 neo by liposome, Expressions of SOCS3 mRNA and protein were detected by RT-PCR and immunocytochemical method, respectively. The expression of c-fos and c-jun mRNA was assessed by semi-quantitative RT-PCR. ~(3)H-TdR incorporation was used to measure the cell proliferation. The SOCS3 expression in A549 cells transfected with SOCS3 gene was confirmed by RT-PCR and immunocytochemical method, respectively. Compared with that in the contrl group, c-fos and c-jun mRNA level decreased significantly in pEFSOCS3-transfected A549 cells (P<0.01). ~(3)-TdR incorporation in pEFSOCS3-transfected A549 cells was significantly lower than that in the control group (P<0.01). SOCS3 protein might decrease the expression of c-fos and c-jun and inhibit the proliferation of A549 cells by regulating STAT3-mediated signal pathway negatively.