2004•Di-san junyi daxue xuebaoRequires access
Effects of transfected SOCS3 gene on expression of c-fos and c-jun mRNA and proliferation of A549 cells
Yu Zu
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Abstract
Objective To explore the effects of transfected SOCS3 gene on the expression of c fos and c jun mRNA and proliferation of A549 cells. Methods A549 cells were cotransfected with pEFSOCS3 and pSV2neo by liposome. Expressions of SOCS3 mRNA and protein were detected by RT PCR and immunocytochemical method, respectively. The expression of c fos and c jun mRNA was assessed by semi quantitative RT PCR. 3H TdR incorporation was used to measure the cell proliferation. Results The SOCS3 expression in A549 cells transfected with SOCS3 gene was confirmed by RT PCR and immunocytochemical method, respectively. Compared with that in the control group, c fos and c jun mRNA level decreased significantly in pEFSOCS3 transfected A549 cells ( P 0 01). 3H TdR incorporation in pEFSOCS3 transfected A549 cells was significantly lower than that in the control group ( P 0.01). Conclusion SOCS3 protein might decrease the expression of c fos and c jun and inhibit the proliferation of A549 cells by regulating STAT3 mediated signal pathway negatively.