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[Effect of Anti-CD44 Monoclonal Antibody A3D8 on Expression of AP-1 in HL-60 Cells].

Jie Li, Jie Yang, Jun Jie Yuan, Yan Li, Rui-Cang Wang, Suyun Wang, Hong-Ling Hao

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Abstract

OBJECTIVE: To explore the effect of anti-CD44 monoclonal antibody A3D8 on expression of transcription factor AP-1 in acute myeloid leukemia cells. METHODS: After acute leukemia cell line HL-60 was treated by different concentrations of A3D8, the proliferation and cell cycle were detected by MTT and FCM respectively. The expressions of c-JUN and c-FOS at mRNA and protein level were detected by RT-PCR and Western Blot respectively. RESULTS: cells. The expressions of c-JUN at mRNA and protein level were both decreased in HL-60 cells treated with A3D8. The expressions of c-FOS at mRNA and protein level in rapamycin treatment groups showed no statistically significant difference as compared with that in control group. CONCLUSIONS: A3D8 can affect the activity of AP-1 through inhibiting the expressions of c-JUN at mRNA and protein level.

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What this paper is about

OBJECTIVE: To explore the effect of anti-CD44 monoclonal antibody A3D8 on expression of transcription factor AP-1 in acute myeloid leukemia cells. METHODS: After acute leukemia cell line HL-60 was treated by different concentrations of A3D8, the proliferation and cell cycle were detected by MTT and FCM respectively. The expressions of c-JUN and c-FOS at mRNA and protein level were detected by RT-PCR and Western Blot respectively. RESULTS: cells. The expressions of c-JUN at mRNA and protein level were both decreased in HL-60 cells treated with A3D8. The expressions of c-FOS at mRNA and protein level in rapamycin treatment groups showed no statistically significant difference as compared with that in control group. CONCLUSIONS: A3D8 can affect the activity of AP-1 through inhibiting the expressions of c-JUN at mRNA and protein level.

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Available abstract

OBJECTIVE: To explore the effect of anti-CD44 monoclonal antibody A3D8 on expression of transcription factor AP-1 in acute myeloid leukemia cells. METHODS: After acute leukemia cell line HL-60 was treated by different concentrations of A3D8, the proliferation and cell cycle were detected by MTT and FCM respectively. The expressions of c-JUN and c-FOS at mRNA and protein level were detected by RT-PCR and Western Blot respectively. RESULTS: cells. The expressions of c-JUN at mRNA and protein level were both decreased in HL-60 cells treated with A3D8. The expressions of c-FOS at mRNA and protein level in rapamycin treatment groups showed no statistically significant difference as compared with that in control group. CONCLUSIONS: A3D8 can affect the activity of AP-1 through inhibiting the expressions of c-JUN at mRNA and protein level.

Key concepts: Molecular biology, Myeloid leukemia, Monoclonal antibody, CD44, Messenger RNA, Western blot, Cell culture, Cell growth

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[Effect of Anti-CD44 Monoclonal Antibody A3D8 on Expression of AP-1 in HL-60 Cells]. — Research Paper | ScholarLens