Quantitative Determinnation of Chlorogenic Acid and Baicalin in Qingkailing Granule by HPLC

Objectve To develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in Qingkailing Granule.Methods A C18(150mm×4.6mm,5μm) bond silica for reersed-phase HPLC was prepared.The mobile phase was CH3OH-0.4% Phosphoric acid.The flow rate was set at 1.0ml/min.The detection wavelength was at 323 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 5.567~139.4μg/mL and 8.640~216.0μg/mL respectively;The correlation coefficient were 0.9995 and 0.9997;The recoveries of adding sample rate were 99.77% and 100.68%;The detection limits were 0.05μg and 0.06μg.The RSD(n=5) of measurement precisions test were 0.99% and 1.45%;The RSD(n=5) of reproducibility between tests were 1.48% and 0.72%.The RSD(n=5) of stability test were 2.53% and 1.45%.The content of chlorogenic acid in compound Qingkailing granule was 0.722~1.086 mg/bag,and that of baicalin was 22.636~23.463 mg/ bag.Conclusion The method is simple,fast,accurate,sensitive,reproducible and low cost.It is fit for the quality control of compound Qingkailing Granule.