2010•Shipin yanjiu yu kaifaRequires access
Mutagenesis Selection of Soybean Protease-producing Strains and Research on Enzyme Characteristics
Qin Li-su
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Abstract
An soybean protease-producing strain D-16 is isolated from market sewer and classified as Bacillus.D-16 produced 1 164.00 U/mL of alkaline protease.By treated the original strain with UV and HNO2,we get the mutant D-16-9.D-16-9 produced 6 518.40 U/mL of alkaline protease,the enzyme activity increased 5.6 times,ability of soy protein hydrolysis also increased 4.12 times.The optimal temperature and pH for the soybean protease are 40 ℃ and pH 10.5.It is stable under 60 ℃ and pH7.0-12.0.Mg2+ could enhance the enzyme activity,while addition of Pb2+,EDTA can greatly inhibit the enzyme activity.