Enzyme properties of alkaline protease produced by marine bacterium strain 15E (Brevibacterium oxydans)

The enzyme properties of alkaline protease from marine bacterium strain 15E(Brevibacterium oxydans) were tested.The alkaline protease has molecular weight 49.0 ku,isoelectric point 9.3.The optimum condition for the protease activity was 60 ℃ and pH 7.0 to 9.0.Ions such as Ca2+,Mg2+ and K+ promoted the activity of the protease.The protease was tolerable to some denaturing agents such as SDS,urea,guanidine HCl,while it was inhibited by PMSF,AEBSF and EDTA,which indicated the alkaline protease belonged to the type of serine protease and some metal ions was important for catalytic activity.