Properties of alkaline protease produced by strain 113.
Gu YanLi, Sainan Liu, Hui Zhang, Xianzhen Li
Abstract
Gu YanLi, Sainan Liu, Hui Zhang, Xianzhen Li
Abstract
[Objective] This study was aimed to investigating properties of alkaline protease produced by strain I13.[Method] Crude enzyme of alkaline protease was obtained from alkaline protease produced by strain I13,while effects of temperature and pH value on enzyme activity was also investigated in this study.[Result] The optimal temperature of alkaline protease produced by strain I13 was 40 ℃,and over 40% of the largest enzyme activity still maintained within the range from 20 to 30 ℃.The optimal pH value was 10.5,and over 90% of the largest enzyme activity still maintained within the range from 8.0 to 11.0,which had broader pH value spectrum.[Conclusion] This alkaline protease has huge potential to be developed into washing-powder additive.
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[Objective] This study was aimed to investigating properties of alkaline protease produced by strain I13.[Method] Crude enzyme of alkaline protease was obtained from alkaline protease produced by strain I13,while effects of temperature and pH value on enzyme activity was also investigated in this study.[Result] The optimal temperature of alkaline protease produced by strain I13 was 40 ℃,and over 40% of the largest enzyme activity still maintained within the range from 20 to 30 ℃.The optimal pH value was 10.5,and over 90% of the largest enzyme activity still maintained within the range from 8.0 to 11.0,which had broader pH value spectrum.[Conclusion] This alkaline protease has huge potential to be developed into washing-powder additive.
Key concepts: Alkaline protease, Protease, Enzyme, Strain (injury), Chemistry, Enzyme assay, Chromatography, Biochemistry