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ANALYSIS OF PYQUITON BY REVERSED PHASE HIGHPERFORMANCE LIQUID CHROMATOGRAPHY

Lin Li

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Abstract

A HPLC method was developed to analyze pyquiton under the following conditions: Column: YWG-CH 10μ φ 5 mm×200 mm; eluent: methanolwater (100:40 v/v); flow rate: 0.5 ml/min; detector: UV-200 Double Beam Spectrophotometer (Shimadzu, Japan); micro flow through cell: 8 μ1 'H' geometry; detecting wavelength: λ_(max) 263 nm, α-asarone was used as internal standard. The recovery is 99～101% and CV1.6% (n=4). This method is more accurate, rapid and simple than the UV-TLC method.

About this research paper

What this paper is about

A HPLC method was developed to analyze pyquiton under the following conditions: Column: YWG-CH 10μ φ 5 mm×200 mm; eluent: methanolwater (100:40 v/v); flow rate: 0.5 ml/min; detector: UV-200 Double Beam Spectrophotometer (Shimadzu, Japan); micro flow through cell: 8 μ1 'H' geometry; detecting wavelength: λ_(max) 263 nm, α-asarone was used as internal standard. The recovery is 99～101% and CV1.6% (n=4). This method is more accurate, rapid and simple than the UV-TLC method.

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Available abstract

A HPLC method was developed to analyze pyquiton under the following conditions: Column: YWG-CH 10μ φ 5 mm×200 mm; eluent: methanolwater (100:40 v/v); flow rate: 0.5 ml/min; detector: UV-200 Double Beam Spectrophotometer (Shimadzu, Japan); micro flow through cell: 8 μ1 'H' geometry; detecting wavelength: λ_(max) 263 nm, α-asarone was used as internal standard. The recovery is 99～101% and CV1.6% (n=4). This method is more accurate, rapid and simple than the UV-TLC method.

Key concepts: Chromatography, High-performance liquid chromatography, Detector, Column chromatography, Volumetric flow rate, Analytical Chemistry (journal), Chemistry, Wavelength

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