HPLC Determination of Related Compounds of Isonine
Chen Wei-jiang
Abstract
Chen Wei-jiang
Abstract
Related compounds of isonine were determinated by HPLC .The analytical column was ODS C18(length:250mm×4.6mm;filler:Hypersil;size:5μm).The mobile phase consisted of methanol and water(10:90)and the flow rate was 1ml.min -1 . The detection was carried out with UV detector and the detection wavelength of 248nm was specified.The proposed method was selective,simple and accurate.
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Related compounds of isonine were determinated by HPLC .The analytical column was ODS C18(length:250mm×4.6mm;filler:Hypersil;size:5μm).The mobile phase consisted of methanol and water(10:90)and the flow rate was 1ml.min -1 . The detection was carried out with UV detector and the detection wavelength of 248nm was specified.The proposed method was selective,simple and accurate.
Key concepts: High-performance liquid chromatography, Chromatography, Methanol, Materials science, Phase (matter), Volumetric flow rate, Detector, Chemistry