Effects of RNA interfering E2F-1 gene expression on biological behavior of gastric cancer cell line MGC803

Objective To investigate the effects of E2F-1-siRNA on biological behaviors of gas-tric adenocarcinoma cell line MGC803 by cDNA microarray. Methods The total RNA was extracted from gastric adenocarcinoma cell line MGC803 transfected with E2F-1-siRNA and negative control-siRNA and then purified. The cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR), and then labeled with Cy5 and Cy3 fluorescence as probes, which were hybridized with gene chip containing 215222 human 22k gene expression profile. Subsequently, the two signal images were scanned by LuxScan 10K/A dual pathways laser scanner and analyzed by LuxScan3.0 image analysis software. Results Of the 215222 target genes, 18 genes were screened out for differences in gene expression level between these two groups. Eight of the 18 genes were up-regulated and 10 were down-regulated,of which one had unknown function respectively. Conclusion After RNA interfering E2F-1 in vitro,the change of the gastric adeno-carcinoma cellular biological behavior is resulted from the interaction between multiple genes and regula-tion in signal pathways. Key genes and signal transduction pathway deserve further investigation. Key words: Gastric carcinoma;  RNA interference;  cDNA microarray;  Gene expression