The molecular mechanism of the effects of E2F-1 overexpression on biological characteristics of human gastric carcinoma cell line

Objective To study the molecular mechanism of the effects of E2F-1 overexpression on biological characteristics of human gastric carcinoma cell line with cDNA microarray technique. Methods Total RNA were extracted from gastric cancer cell line MGC-803 transfected with E2F-1 and then purified. The cDNA obtained by reverse transcription polymerase chain reaction (RT-PCR),was labeled with Cy5 and Cy3 fluorescence as probes,and then hybridized with gene chip containing 21522 human 22K gene expression profile. Subsequently,the two signal images were scanned by LuxScan 10K/A dual pathways laser scanner and analyzed by LuxScan3.0 image analysis software. We randomly chose three of the differentially expressed genes to confirm the array results using semi-quantitative RT-PCR. Results Of the 21522 target genes,740 genes were screened out for differences in gene expression level. 405 of the 740 genes were up-regulated and 335 were down-regulated. 73 of differentially expressed genes were with unknown function. The results of RT-PCR were well coincided with the cDNA microarray results. Conclusion The carcinogenesis of gastric carcinoma includes the interaction of multiple genes and regulation of many signal transduction pathways. E2F-1 can affect the biological characteristics of MGC-803 by TP53 signal pathway,and is closely associated with the differentially expressed genes.