Comparison of rat liver cold ischemia/reperfusion injury model with portosystemic shunt and liver transplantation model

Objective To establish a rat model of in situ cold ischemia/reperfusion (I/R) with portosystemin shunt and compare to liver transplantation model.Methods Wistar rats were divided into three groups:group A (n =10),control group; group B (n =140),orthotopic liver transplantation group; and group C (n =94),liver cold ischemia group with splenic transposition (LCI).The parameters measured included portal venous pressure (PVP),liver enzymes [(total bilirubin (TBIL),alanine aminotransferase (ALT),alkaline phosphatase (ALP) and GGT),and 14-day survival rate.Results Both groups showed characteristic I/R histologic changes at 24th h and 7th day.PVP at 1 min and 10 min after porta hepatis clamping in group B was significantly higher than that in group C [1 min:(29.93 ± 2.42) mm Hg vs.(22.91 ±4.01) mmHg,P＜0.01; 10 min:(57.12 ±4.44) mmHgvs.(18.32 ±4.42) mmHg,P ＜0.01].TBIL,GGT and ALP were higher in groups B and C than in group A on the postoperative day (POD) 0 (P＜0.05).TBIL,GGT,ALT and ALP were higher in groups B and C than in group A on POD 1 (P ＜ 0.05),and the index was higer in Group B than group C on POD 1 (P ＜ 0.05).GGT was still higher in group B than in groups C and A on POD 7 [groups B and A:(2.16 ±0.34) IU/L vs.(1.39 ± 0.36) IU/L; groups B and C:(2.16 ±0.34) IU/L vs.(1.75 ±0.32) IU/L; P＜0.05 for both].The 14-day survival rate in group C was higher than in group A (P ＞ 0.05).Conclusion LCI model is ideal for the study on cold I/R injury. Key words: Liver;  Cold ischemia/reperfusion;  Model, animal