姜树林, 孙树秋, 姚洪菊, 孙殿军
Abstract
目的观察砷、氟及砷氟联合染毒对SGC-7901细胞基因组DNA的损伤作用及特点.方法砷、氟染毒剂量10 μmol/L,染毒后4 h应用单细胞凝胶电泳技术结合彗星图像分析系统检测DNA损伤.结果①NaAsO2组尾长和尾动量明显大于对照组,NaF组拖尾率显著高于对照组;②氟砷联合染毒组拖尾率、尾DNA含量、尾长和尾动量与对照组差异均有显著意义,但氟与砷引起的DNA损伤作用之间没有观察到交互作用.结论 NaAsO2与NaF均可引起DNA损伤,但它们的损伤机理可能是不同的;氟与砷联合染毒仅表现为简单相加作用.
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目的观察砷、氟及砷氟联合染毒对SGC-7901细胞基因组DNA的损伤作用及特点.方法砷、氟染毒剂量10 μmol/L,染毒后4 h应用单细胞凝胶电泳技术结合彗星图像分析系统检测DNA损伤.结果①NaAsO2组尾长和尾动量明显大于对照组,NaF组拖尾率显著高于对照组;②氟砷联合染毒组拖尾率、尾DNA含量、尾长和尾动量与对照组差异均有显著意义,但氟与砷引起的DNA损伤作用之间没有观察到交互作用.结论 NaAsO2与NaF均可引起DNA损伤,但它们的损伤机理可能是不同的;氟与砷联合染毒仅表现为简单相加作用.
Key concepts: DNA damage, Fluoride, Chemistry, Arsenic, DNA, Molecular biology, Biochemistry, Cell biology