2013•Zhonghua fangshe yixue yu fanghu zazhiRequires access
Radiosensitive effect of TRAIL on esophageal cancer cell line-Eca109
Lili Zhang, Xiguang Liu, Hao Song, Hongjun Zhang, Yukun Wang
Open publisher page 0 citations
Abstract
Objective To study the radiosensitive effect of Apo-2 ligand (Apo2L),known as tumor necrosis factor-related apoptosis inducing ligand (TRAIL),on esophageal cancer cell line-Ecal09 in vitro.Methods MTT assay and clonogenic assay were performed to evaluate the cytotoxicity and radiosensitization of TRAIL,respectively.Cell apoptosis and cell cycle distribution were measured by flow cytometry (FCM).Results TRAIL inhibited cell growth in a dose-dependent manner and its 50％ inhibition concentration(IC50) was 637 μg/ml.The concentration 200 μg/ml TRAIL could enhance cell radiosensitivity with a SER of 1.219.After 2,4,6,8 Gy X-ray irradiation,the incidence of apoptosis in TRAIL-treated cells was higher than that of irradiation alone groups(F =16.97,76.65,92.86,209.66,P ＜0.05).The percentage of cells in G2/M phase of TRAIL-treated groups was also higher than that of irradiation alone groups (F =9.40,84.99,87.61,2025.85,P ＜ 0.05).Conclusions TRAIL could inhibit cell growth,induce apoptosis and G2/M arrest,and had radiosensitization effect on Eca-109 cells.
Key words:
TRAIL; Esophageal cancer cell; Radiosensitivity ; Apoptosis ; G2/M Arrest