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Proliferation and Apoptosis of Human Lung Cancer Cell Line A549

Feng Chen

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Abstract

ObjectiveTo study the effect of As2O3 and TRAIL on A549 cell proliferation and apoptosis.MethodsHuman lung cancer cell line A549 was treated with As2O3 and TRAIL at different concentrations in vitro.The inhibitory effect was observed by MTT assay.Hoechest staining was applied to analyze the apoptosis effect.Flow cytometry with PI staining was used to detect the apoptosis rate and cell cycle after treatment.ResultsDifferent concentrations of As2O3 combined TRAIL had more stronger inhibitory effect on A549 cells than As2O3 or TRAIL.After As2O3 and TRAIL were applied for 48 h,more apoptosis cells could be seen than As2O3 or TRAIL by Hoechest staining and flow cytometry;the percentage of G2/M were higher.ConclusionAs2O3 in combination with TRAIL can synergistic inhibit the proliferation of A549 in vitro and induce apoptosis,and the mechanism is related to blockade of A549 cell in G2/M cycle.

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ObjectiveTo study the effect of As2O3 and TRAIL on A549 cell proliferation and apoptosis.MethodsHuman lung cancer cell line A549 was treated with As2O3 and TRAIL at different concentrations in vitro.The inhibitory effect was observed by MTT assay.Hoechest staining was applied to analyze the apoptosis effect.Flow cytometry with PI staining was used to detect the apoptosis rate and cell cycle after treatment.ResultsDifferent concentrations of As2O3 combined TRAIL had more stronger inhibitory effect on A549 cells than As2O3 or TRAIL.After As2O3 and TRAIL were applied for 48 h,more apoptosis cells could be seen than As2O3 or TRAIL by Hoechest staining and flow cytometry;the percentage of G2/M were higher.ConclusionAs2O3 in combination with TRAIL can synergistic inhibit the proliferation of A549 in vitro and induce apoptosis,and the mechanism is related to blockade of A549 cell in G2/M cycle.

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Available abstract

ObjectiveTo study the effect of As2O3 and TRAIL on A549 cell proliferation and apoptosis.MethodsHuman lung cancer cell line A549 was treated with As2O3 and TRAIL at different concentrations in vitro.The inhibitory effect was observed by MTT assay.Hoechest staining was applied to analyze the apoptosis effect.Flow cytometry with PI staining was used to detect the apoptosis rate and cell cycle after treatment.ResultsDifferent concentrations of As2O3 combined TRAIL had more stronger inhibitory effect on A549 cells than As2O3 or TRAIL.After As2O3 and TRAIL were applied for 48 h,more apoptosis cells could be seen than As2O3 or TRAIL by Hoechest staining and flow cytometry;the percentage of G2/M were higher.ConclusionAs2O3 in combination with TRAIL can synergistic inhibit the proliferation of A549 in vitro and induce apoptosis,and the mechanism is related to blockade of A549 cell in G2/M cycle.

Key concepts: Apoptosis, A549 cell, Flow cytometry, Cell cycle, Cell growth, MTT assay, Cell culture, Cell

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