Prediction of acute heart transplant rejection in rats with protein spectrum technique

Objective To study the prediction of acute heart transplant rejection in rats with protein spectrum technique.Methods The xenogeneic rat cervical heterotopic heart transplantation model was set up.( 1 ) The donors and recipients of acute rejection groups A and B were not subjected to pre-treatment; (2) In treatment groups A and B,receptors were subjected to intraperitoneal injection of cyclosporine A (CsA) 20 mg/kg one day preoperation,10 mg/kg per day postoperation.At 5th and 7th day postoperation,blood samples and the graft (donor hearts) specimens were collected; (3) In control group,blood and myocardial tissue specimens were taken from the healthy Wistar rats before transplantation.By using proteomic technologies,serum samples and the myocardial tissue samples of protein in all groups were measured.The differential proteins among group were analyzed and the peak intensity of the differential proteins was compared.Results As compared with the serum samples of control group,there were 7 differential protein peaks in acute rejection group A,and 11 differential protein peaks in acute rejection group B.The comparison between acute rejection group A and treatment group A revealed that there were 4 differential protein peaks.The comparison between acute rejection group B and treatment group B showed that there were 6 differential protein peaks.For myocardial proteins in the control group compared to acute rejection groups A and B,there were 7 and 13 differential protein peaks,respectively.Above comparisons were statistically significant ( P ＜ 0.01 ).Conclusion Differential proteins in acute rejection at different time points exist,wich my be related with acute rejection. Key words: Heart transplantation;  Acute rejection;  Protein spectroscopy