Relationship between the length and site of intestinal grafts and the results of rejection in rats

Objective To explore the relationship between the results of rejection and the length and site of intestinal grafts.Methods Allogeneic orthotopic intestinal transplantation was performed (SD→Wistar).The recipients received 30% proximal intestine (group A),60% proximal intestine (group B),total intestine (group C) and 30% distal intestine (group D) respectively.Survival time was observed.On the day 1,3,5,7,9 after operation,the blood samples were obtained to monitor the change of serum IL 2,IL 8.Maltose absorption tests were performed.The grafts were harvested and subjected to histologic examination with H E staining.Results The survival time was significantly different between group A (12.00± 1.67) days,B (11.17± 1.94) days and group C (8.33± 1.03) days,D (9.17± 1.17) days ( P 0.05). Maltose absorption tests,serum and histologic findings showed mild acute rejection occurred on day 3 after operation in groups C,D and on day 5 in groups A,B;Moderate acute rejection occurred on day 5 in groups C,D and on day 7 in groups A,B;Severe acute rejection occurred on day 7 in groups C,D and on day 9 in groups A,B.Conclusion The results of rejection have no direct relationship with the length of the grafts.The time of onset of the rejection have some relationship with the site of intestinal grafts.