Establishment of acute renal rejection model in rats after kidney transplantation

Objective To establish the model of acute renal rejection in rats after kidney transplantation and investigate the pathomechanism of transplanted tissue.Methods Seventy-eight normal male SD rats were divided into normal group(group A,n=15),pseudo-surgery group(group B,n=15),non-acute graft rejection group(group C,n=16,SD rats as donor),and acute graft rejection group(group D,n=16,Wistar rats as donor).The renal function of these four groups was evaluated at the seventh day after transplantation operation.Grafts were harvested at the seventh day and were observed the pathological changes under the light microscope.Acute rejection semiquantitative scores were analyzed according to the standard of Banff 97.Results The serum levels of urea nitrogen,creatinine and acute rejection semiquantitative scores were significantly higher in group C and group D than those in group A(P0.05),and were higher in group D than those in group C(P0.01).Conclusion Wistar-SD rats kidney transplantation can be used for acute rejection model after kidney transplantation.