Expression of extracellular matrix metalloproteinase inducer CD147 and matrix metalloproteinase-9 in gallbladder carcinoma and precancerous lesions

Objective To investigate the expression of extracellular matrix metalioproteinase inducer CD147 and matrix metalloproteinase-9 (MMP-9) in the gallbladder carcinoma and precancerous lesions and their correlation with occurrence,progress,and prognosis of gallbladder carcinoma.Methods Streptavid-in-peroxidase (SP) immunohistochemistry was used to detect the expression of CD147 and MMP-9 in surgically resected specimens of 42 gallbladder carcinomas,18 gallbladder epithelial atypical dysplasia Ⅱ-Ⅲ tissue samples,13 gallbladder epithelial atypical dysplasia Ⅰ tissue samples,15 gallbladder adenomas and 15 chronic cholecystitis tissue samples.Results The positive rate of CD147 (83.33％)and MMP-9 (88.10％) proteins was higher in gallbladder carcinomas than in gallbladder epithelial atypical dysplasia Ⅰ tissues (38.46％,30.77％),gallbladder adenomas (20.00％,26.67％) and chronic cholecystitis tissues (6.67％,20.00％) (P ＜0.01),but no significant difference was found in comparison to gallbladder epithelial atypical dysplasia Ⅱ-Ⅲ tissues (61.11％,66.67％,P ＞0.05).The expression of CD147 and MMP-9 was positively correlated with the tumor differentiation,Nevin staging and lymph node metastasis (P ＜0.05).The CD147 expression was positively correlated with MMP-9 expression in gallbladder carcinoma (rs =0.427,P ＜0.01).Patients negative for the expression of CD147 and MMP-9 had longer survival time than those with positive expression of CD147 and MMP-9.Conclusion CD147 and MMP-9 play important roles in carcinogenesis of gallbladder mucosa,and invasion,metastasis and prognosis of gallbladder carcinoma. Key words: Gallbladder carcinoma; Atypical dysplasia; CD147; Matrix metalloproteinase