Three different modified methods for establishment of middle cerebral artery occlusion model in rats

Objective To compare and evaluate the three different modified methods for establishment of middle cerebral artery occlusion model in rats.Methods Sixty male SD rats were randomly divided into group A,group B,and group C (n =20 each).The different methods were adopted to establish the middle cerebral artery occlusion models in the three groups.The success rate of model construction,postoperative mortality within 72 h,mean operative time and suture inserting time of each group were determined.72 h after operation,the brain pathological changes of alive rats were observed.Results The postoperative mortality within 72 h in group C was 5％,mean operative time was (18.20 ± 0.99) min,and mean suture inserting time was (3.05 ± 0.23) min,all significantly decreased as compared with groups A and group B (all P ＜ 0.05).The success models of each group shown pathological changes of cerebral infarction.Conclusion The modified method of group C reduces the operating difficulty,shortens the operating time,and can establish model of focal cerebral ischemia in rats more rapidly and effectively. Key words: Middle cerebral artery occlusion;  Rat;  Model, animal