The Improvement of Focal Cerebral Ischemia Model of Permanent Middle Cerebral Artery Occlusion Induced by Suture Method

Objective To study the worth of focal cerebral ischemia model of permanent middle cerebral artery occlusion(PMCAO) induced by suture method improved from the classic method.Methods SD rats were randomly divided into three groups:normal control group(n=8);sham-operated group(n=8);cerebral ischemia group,in which the rats were divided into 6 h or 24 h cerebral ischemia group(n=8 for each).Cerebral infarction area was assessed by staining with 2% 2,3,5-triphenyltetrazolium chloride(TTC) solution and pathological changes of cerebral infarction was assessed by staining with hematoxylin-eosin(H-E).Results Cerebral infarction area continuously increased and pathological changes of cerebral infarction continuously exacerbated at ischemic hemisphere from 6 h to 24 h after middle cerebral artery occlusion,while similar changes did not found in normal control and sham-operated rats.In additional,to use this method,90% of them is successful.Conclusion The modified PMCAO model can be operated simply and is reliable,stable and repeatable,and cerebral infarction is consistent with the clinical pathological process.It is perfect PMCAO model to study focal cerebral ischemic injury.