Surveillance of group A rotavirus diarrhea in children under 5 years old in Beijing,2011

Objective To investigate the epidemiological characteristics of group A rotavirus diarrhea in children under 5 years old in Beijing.Methods Stool specimens of 604 children with diarrhea,who have got infusion twice and more,were collected from three hospitals in Beijing throughout 2011.The specimens were detected by ELISA,and the positive samples were genotyped using semi-nested reverse transcription PC R.Results A total of 96 positive cases were detected among 604 patients with diarrhea,with the positive rate of 15.89％.Group A rotavirus diarrhea was most common during autumn and winter,and the highest positive rate was in November (55.56％,30/54).Rotavirus diarrhea most occurred in 3 ～ 23-month-old infants ( 93.76％,90/96 ).All the positive specimens were genotyped for G/P.G3 and G9 mixed infection rank first (37.50％,36/96),followed by G3 (28.12％,27/96),Gl (11.46％,11/96),G9 (9.38％,9/96),G2 (8.33％,8/96).P8 type was the most dominant (86.46％,83/604),and the second was P4 (10.42％,10/96).G3 + G9/P [ 8 ] was the most common G/P combination (36.46％,35/96),followed by G3/[P8] (26.04％,25/96),G1/[P8] (11.46％,11/96),G9/[P8] (9.38％,9/96),G2/[P4] (8.33％,8/96).Conclusion Rotavirus was one of the main pathogens for infantile diarrhea,and the major epidemic strains was G3 + G9/P[ 8 ]. Key words: rotavirus; young children; diarrhea; genotype