2019•Chin J Obes Metab Dis(Electronic Edition)Requires access
Effects of miR-1908 on cell proliferation, cell cycle, apoptosis of human preadipocytes-visceral
郭冰冰, 付锦艳, 裴晶晶, 蒋新液
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Abstract
Objective
To study the effect of miR-1908 on cell proliferation,cell cycle,apoptosis of human preadipocytes-visceral.
Methods
human preadipocytes-visceral were cultured and divided into three treatment groups—inhibitor group (transfected with chemically modified miR-1908-5p inhibitor), empty vector group (transfected empty carrier) and control group (without any treatment).CCK8 assay was used to detect the proliferation of HPA-v, Tunnel method was used to detect the apoptosis, Flow cytometry was used to detect cell cycle.
Results
CCK8 assay showed that the absorbance values of miR-1908 inhibitor group (0.61±0.07) was significantly lower than that of the control group (1.01±0.05) (P<0.05). Tunel results showed that the apoptotic rate of miR-1908 inhibitor group (0.27±0.03)% was significantly higher than that of control group (0.13±0.05)% (P<0.05). The results of flow cytometry showed that miR-1908 inhibitor might induce the G0/G1 phase cell cycle arrest.
Conclusions
Inhibition of miR-1908 expression could significantly inhibit HPA-v cell proliferation, induced apoptosis and change the cell cycle distribution.
Key words:
MiR-1908; Adipocytes; Proliferation; Apoptosis; Cell cycle