The effect of mir-15a and mir-16-1 on the apoptosis of Raji lymphomatic cells

Aim:To explore the apotosis effects of mir-15a and mir-16-1 in inducing Raji cell line. Methods: mir-15a and mir-16-1 oligonucleotides、control group were all transfected into Raji cells with lipofectamine 2000.Bcl-2 protein expression of every group cells was detected by indirect immunofluorescence;Semi-quantitative RT-PCR detected the expression level of Bcl-2 mRNA;The growth inhibitory effect of Raji cells was measured by trypan blue dye exclusion method and CCK8 assay.The apoptotic cells were observed by Hoechst Method;Flow Cytometry(FCM)—AnnexinV/PIdouble dyeing method was used to detect the cell apoptotic rate.Results: After Raji cells were transfected for 48 h,Bcl-2 protein expression levels of mir-15a group and mir-16-1 group cells obviously decreased,The above two groups had obviously difference as compared with the control group and blank group (P0.05).The expression of Bcl-2 mRNA among the four groups had no obviously difference,.Trypan blue dye exclusion method and CCK8 assay showed that transfection of mir-15a and mir-16-1 decreased the cell growth at 24,48 and 72 h post-transfection,apoptotic cells can be seen with Hoechst Method at 48 h after being transfected.FCM assays indicated that the cell apoptotic rates in earlier period and advanced stage of mir-15a group were 9.74% and 9.65%,and mir-16-1 group were 9.70% and 9.34%,which were obviously higher than blank group and control group.Conclusion:mir-15a and mir-16-1 can induce apoptosis of lymphomatic cells.