2009•Zhonghua shiyan waike zazhiRequires access
Selective inhibitory effects of artemisinin and holotransferrin on human hepatocellular carcinoma SMMC-7721 cells
Xiaorong Deng, Lei Pan, Heping Yu
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Abstract
Objective To investigate the selective inhibitory effect of artemisinin and holotransferrin on human hepatoeellular carcinoma SMMC-7721 cells in vitro.Methods MTT and trypan blue assay were used to assess the proliferation and viability of SMMC-7721 eeUs treated with artemisinin and holotransferrin.The inhibition of colony formation elicited by artemisinin was determined by colony formation assay.Results Artemisinin significantly inhibited SMMC-7721 cell growth,colony formation and cell viability at various concentrations (P < 0.05).It was found that combined incubation with holotransferrin, which increased the concentration of ferrous iron in cancer cells, sensitized the growth inhibitory effect of artemisinin on SMMC-7721 cells (P < 0.01).Conclusion Artemisinin and holotransferrin effectively inhibited SMMC-7721 cell viability, growth, and proliferation, which may provide a potential therapeutic choice for liver cancer.
Key words:
Carcinoma, hepatoeellular; Artemisinin; Transferrin; Cell growth; Cell via-bility