The expression of human runt-related transcription factor 3 and Cyclin D1 mRNA in pancreatic carcinoma and their correlation with clinicopathologic features

Objective To study the expression of human runt-related transcription factor 3 (RUNX3) and Cyclin D1 mRNA in pancreatic carcinoma and their correlation with the clinicopathologic features.Methods The expression levels of RUNX3 and Cyclin D1 mRNA were detected by using reverse transcription polymerase chain reaction (RT-PCR) in 42 cases of pancreatic carcinoma tissues and their surrounding normal tissues.Results ( 1 ) The relative expression level of RUNX3 mRNA in 42 cases of pancreatic carcinoma tissues was 0.2469 ±0.0708,and 0.7091 ±0.1764 in 42 cases of surrounding normal tissues.There was significant difference in the RUNX3 expression between two kinds of samples (t =15.7036,P ＜ 0.01 ).The expression of RUNX3 mRNA in pancreatic carcinoma was correlated to histologic grade with clinical staging and lymph node metastasis ( P ＜0.05 ),but not to age and sex; (2) The relative expression level of Cyclin D1 mRNA in 42 cases of pancreatic carcinoma tissues was 0.7769 ±0.1456,and 0.2860 ± 0.1224 in 42 cases of surrounding normal tissue.There was significnat difference in the Cyclin D 1 expression between two kinds of samples ( t =18.9158,P ＜ 0.01 ).The expression of Cyclin D1 mRNA in pancreatic carcinoma was correlated to histologic grade with clinical staging and lymph node metastasis (P ＜ 0.05 ),but not to age and sex; (3) The expression of RUNX3 was correlated with Cyclin D1 (r =-0.320,P ＜0.05).Conclusion The expression of RUNX3 mRNA was weak and that of Cyclin D1 mRNA was high in pancreatic carcinoma.They may play an important role in the carcinogenesis and progression of pancreatic carcinoma. Key words: Pancreatic carcinoma;  Runt-related transcription factor 3 gene;  Cyclin D1 gene