miRNA-10b promote proliferation and invasion of low metastasis of lung cancer cell line 95-C

Objective To investigate the effects of miRNA-10b on proliferation,cell cycle,apoptosis,invasion,and migration of low metastasis of lung cancer cell line (95-C).Methods Under the induction of Lipofectamine TM 2000,the recombinant of miRNA-10b was transfected into 95-C.The experiment set up three groups:blank control group,empty vector transfected group,and miRNA-10b expression plasmid transfected group.Transfection effciency was observed by fluorescence microscope.miRNA-10b expression level was detected by real-time PCR after transfection.The cell proliferation was detected by cell proliferation assay.The cell cycle and apoptosis were detected by flow cytometry.The invasive ability of cell was detected by Transwell experiment.The migration ability of cell was detected by the wound healing assay in each group.Results Compared with the blank control group and the empty vector transfected group,the cell proliferation rate was obviously increased,the cell apoptosis rate decreased,the cell invasion and migration ability enhanced after transfection (P ＜0.05).Conclusions miRNA-10b can promote the cell proliferation,reduce the apoptosis,and increase the cell invasion and migration ability of 95-C. Key words: MicroRNA-10b;  95-C;  Proliferation;  Cell cycle;  Apoptosis;  Invasion;  Migration