Effect of miR-199a-5p transfection on the growth of clear-cell renal-cell carcinoma 786-0 cells

Objective MiRNA is an important regulating factor in tumors. Our previous findings showed that the expression of miR-199a-5p was significantly down-regulated in clear-cell renal-cell carcinoma( CCRCC),suggesting an inhibiting effect of miR-199a-5p on tumor growth. This study aims to investigate the role of miR-199a-5p in the proliferation,apoptosis,invasion and cell cycle of CCRCC 786-0 cells. Methods Using liposome-mediated gene transfection,we transfected human CCRCC 786-0 cells with miR-199a-5p mimics( experimental group) and negative control. Then we detected the proliferation of the transfected cells by cell counting kit 8,their invasion ability by transwell assay,and their apoptosis and cell cycles by flow cytometry. Results Compared with the negative control,the 786-0 cells showed significantly increased proliferation ability( P 0. 05) and reduced invasion ability( P 0. 01) in the experimental group,but no obvious differences in apoptosis and cell cycles. Conclusion miR-199a-5p is capable of weakening the invasion ability of human CCRCC 786-0 cells in vitro.