Effects of RNA interference (RNAi) on vascular endothelial growth factor 165 expression in A549 cells

Objective To investigate whether RNA interference (RNAi) induced by small inter-ference RNA (siRNA) could suppress vascular endothelial growth factor (VEGF) 165 expression in non-small lung carcinoma (NSCLC) cells. Methods A549 cells were transfected with chemically synthesized siRNA formulated with RNAiFectTM Transfeetion Kit. The VEGF165 expression was detected by Western blot. The viability of cells was determined by MTT and cell counting. Results Sequence specific siRNAs targeting VEGF165 down-regulated VEGF165 expression significantly after A549 cells were interfered for 24 h. Relative grey scales of control group and interference group after 24,48 and 72 h were 0.96±0.27 vs 0.81±0.10,0.52±0.12 and 0.36±0.10,respectively. There was no effect on the viability of cells (data not shown). Conclusion Sequence specific siRNAs targeting VEGF165 was capable of suppressing the VEGF165 expression. Key words: RNA interference; Vascular endothelial growth factor; Carcinoma, non small lung