Influence of RNA interference on expresssion of vascular endothelial growth factor in human ovarian cancer cells and on cell proliferation

Objective:To investigate the inhibitory effect of vector-based RNA interference(RNAi) on the expression of vascular endothelial growth factor(VEGF) protein in ovarian cancer cells and on proliferation of overian cancer cells. Methods: A vector for transcribing specific small hairpin RNA(shRNA) targeting VEGF gene was constructed,introduced into ovarian cancer HO-8910 cells by Lipofectamine 2000 and the positive clones were screened by G418. The VEGF protein and mRNA expression level of HO-8910 cells were detected by Western blot and semi-quantitative RT-PCR. MTT method and FCM analysis were used to observe the effect of RNAi on proliferation of ovarian cancer cells. Results: The vector of RNA interference was successfully constructed and VEGF expression was descreased significantly in HO-8910-H1-VEGF cells. The growth of ovarian cancer cells transfected with specific small hairpin RNA expression vector was blocked, with G_(1) phase cells increased and S phase cells decreased. Conclusion: The specific small hairpin RNA targeting VEGF mRNA can inhibit the expression of VEGF and the proliferation of ovarian cancer cells, which provides an experimental basis for treating human tumors with anti-angiogenesis method using RNAi. -