2009•Zhongguo pu-wai jichu yu linchuang zazhiRequires access
Study of Inhibition Effects of Small Interfering RNA on VEGF Gene Expression in HepG2 Hepatic Cancer Cell
Jianxin Wang
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Abstract
Objective To observe the effect of RNA interference(RNAi) on HepG2 hepatic cancer cell by small interfering RNA(siRNA).Methods siRNA targeting vascular endothelial growth factor(VEGF) gene was transfected into HepG2 cells by LipofectimineTM 2000.The VEGF mRNA and protein were respectively detected by real-time quantitive PCR and Western blot,and the concentration of VEGF protein in the cell culture supertant was determined by ELISA at 48 h after culture.Results The average efficiency of siRNA transfection was(90.4±2.9)% after 6 h cell culture.The expressions of VEGF mRNA and protein in HepG2 cells could be effectively suppressed by siRNA,and the concentration of VEGF protein in the cell culture supertant was also decreased.Conclusion siRNA can knock down the expression of VEGF gene and decrease the concentration of VEGF protein in HepG2 cells.