Reason analysis of RhE antigen double group and its effect on immune function after blood transfusion

Objective To analyze the cause of RhE antigen double group and investigate the effect on immune function in patients with blood transfusion. Methods The results of blood group identification were collected from 25 069 cases in Henan Provincial People’s Hospital from January 2014 to December 2017. Among them, 2 405 cases were Rh E antigen double group, and the causes of double groups of E antigen were analyzed. Among them. 100 Rh E antigen negative patients were set as group A, and 100 RhE antigen double group patients as group B. The blood donors in both groups were RhE-positive. The incidence of transfusion reaction after blood transfusion in the two groups was tracked. Flow cytometry was used to compare the changes of CD4 + and CD8 + cells before and after transfusion in two the groups. Results The proportion of Rh E antigen positive and Rh E antigen negative was almost the same, Rh E antigen double group accounted for 9.5%. The history of blood transfusion was the main reason of double groups of RhE antigen. The relative risk of adverse transfusion reaction in group B was higher than that in group A; CD4+ T cells increased and CD8+ T cells decreased in group A and group B 5 days after transfusion, but CD4 + and CD8 + cells gradually returned to the normal level 10 days after transfusion in group A, and there was no significant difference between 10 days after transfusion and 5 days after transfusion in group B. The number of CD4 + cells in group B was higher than that in group A 5 and 10 days after transfusion, and the number of CD8 + cells was lower than that in group A (all P<0.05). Conclusions Patients with Rh E antigen double group should be infused with RhE antigen negative blood as far as possible in transfusion strategy to ensure the safety of blood transfusion. Key words: Rh E antigen; Blood transfusion; Immune function