Pathogenic bacteria distribution and drug resistance analysis of 960 patients by means of sputum culture

Objective To observe and analyze the distribution and drug resistance of pathogenic bacteria in respiratory tract of the patients,so as to provide basis for reasonable selection of clinical drugs.Methods Nine hundred and sixty sputum specimens of the hospitalized patients were selected as the research objects.The specimens were conventionally cultured as well as the bacteria were identified and the drug sensitivity test was employed.Results Among the 960 cases of sputum specimens,a total of 685 strains of pathogens were isolated and the detection rate was 71.4％.There were 475 strains of gram negative bacilli,accounting for 69.3％ and there were 136 strains of gram-positive cocci,accounting for 19.9％.Seventy-four strains of fungi were detected,accounting for 10.8％ ; the results of the drug sensitivity analysis showed the gram-negative bacilli were more highly sensitive to the antibiotics such as imipenem,while the gram positive cocci drugs were more highly resistant to the antibiotics such as erythromycin.The resistance rates of the main pathogens to imipenem,vancomycin and linezolid all were 0 ; in Klebsiella,62 strains produced ESBLs and the enzyme production rate was 36.9％.In Escherichia coli,52 strains produced ESBLs and the enzyme production rate was 65％.In Staphylococcus aureus,19 strains were methicillin resistant Staphylococcus aureus,accounting for 36.5％.While in coagulase-negative Staphylococcus,23 strains were methicillin resistant coagulase negative staphylococcus,accounting for 69.7％.Conclusions The results of sputum culture showed that,in the lower respiratory tract infection,the main pathogens were the gram-negative bacilli such as Klebsiella pneumoniae,which are sensitive to the antibiotics such as imipenem,followed by the gram-positive cocci such as Staphylococcus aureus,which are sensitive to the antibiotics such as vancomycin and linezolid.The resistance of Escherichia coli and coagulase negative Staphylococcus aureus resistance are strong.The reasonable use of antibiotics in the clinical practices should be based on the pathogenic bacteria distribution and the results of the drug sensitivity test. Key words: Sputum culture;  Strain identification;  Drug sensitivity test;  Drug resistance rate